HPLC Experiment and Analytic Chemistry Knowledge Write an experimental part and discuss the result of HPLC experiment “please don’t assign for this question if you don’t have analytic chemistry knowledge”
1-write an experimental part of HPLC experiment following “4.” format that in the attachment we did the procedure on page 348, please do not forget that info about the instrument we used.
-Hewlett-Packard HPLC HP Series 1100
-Column: C18 reverse phase, 5-micrometer particle size, 50 mm x 4.6 mm
-Detector: variable wavelength (set to 254 nm)
-Integrator: HP 6890
-Our analysis uses the (aqueous) phosphate buffer instead of the water, so the mobile phase is phosphate buffer/methanol
2-discuss ALL the result of HPLC experiment and make sure to find these two things:
- a) The concentration (mg/L) of caffeine in the original beverage that you calculated from the chromatogram.
- b) compare results obtained above with those on beverage container in both concentration (mg/L) AND total caffeine content